Approaches to altering particle distributions in cryo-electron microscopy sample preparation
نویسندگان
چکیده
منابع مشابه
An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology.
Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances i...
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Cryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the structure of proteins and macromolecular complexes without the need for crystals. Recent advances in detector technology and software algorithms now allow images of unprecedented quality to be recorded and structures to be determined at near-atomic resolution. However, compared with X-ray crystallography, c...
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Recent advances in cryo-electron microscopy have led to an increasing number of high (3-5A) to medium (5-10A) resolution cryoEM density maps. These density maps contain valuable information about the protein structure but frequently require computational algorithms to aid their structural interpretation. It is these hybrid approaches between cryoEM and computational protein structure prediction...
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Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (i.e., unordered, nonaggregated) form is a new approach to the study of molecular assemblies, which are often too large and flexible to be amenable to X-ray crystallography. New insights into biological function on the molecular level are expected from cryo-EM applied to the study of such complexes "trapped" at differ...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section D Structural Biology
سال: 2018
ISSN: 2059-7983
DOI: 10.1107/s2059798318006496